RESTRICTION ENZYME- MODE OF ACTION AND NOMENCLATURE
Restriction Enzymes DNases which follow up on explicit positions or groupings on the DNA are called as restriction endonucleases. The arrangements which are perceived by the restriction endonucleases or restriction enzymes are called as acknowledgment successions or limitation locales. These arrangements are palindromic successions. Diverse limitation chemicals present in various microscopic organisms can perceive extraordinary or same limitation destinations. Be that as it may, they will cut at two distinct focuses inside the limitation site. Such limitation chemicals are called as isoschizomers. Curiously no two limitation compounds from a solitary bacterium will cut at a similar limitation site. Method of activity The restriction enzyme ties to the acknowledgment site and checks for the methylation (nearness of methyl bunch on the DNA at a particular nucleotide). In the event that there is methylation in the acknowledgment grouping, at that point, it just tumbles ...