GENE CLONING
Gene cloning A clone is an exact copy of an organism, organ, single cell, organelle or macromolecule. Gene cloning is the act of making copies of a single gene. Molecular cloning refers to the procedure of isolating a defined DNA sequence and obtaining multiple copies of it in vivo. Cloning is frequently employed to amplify DNA fragments containing genes, but it can be used to amplify any DNA sequence such as promoters, non-coding sequences, chemically synthesised oligonucleotides and randomly fragmented DNA. Cloning is used in a wide array of biological experiments and technological applications such as large scale protein production. It is used in many areas of research and for medical applications such as gene therapy. Selective amplification of genes depends on the ability to perform the following essential procedures. 1. Amplification of a specific gene The discovery of thermostable DNA polymerases, such as Taq Polymerase, made it possible to manipulate DNA replication in the...