AGROBACTERIUM TUMEFACIENS- GENE TRANSFER
AGROBACTERIUM TUMEFACIENS Agrobacterium is a gram-negative bacteria involved in causing root gall formation disease in plant species. The crown gall formation is due to the transfer of a segment of oncogene DNA into plant cell at wounded sites. This DNA segment i.e. T-dna or transfer DNA is present on a large plasmid called tumor-inducing plasmid in the bacterium. The T-dna is integrated into the plant chromosome by recombination. I series of vir genes are involved in directing this infection process. So when the plant root or stem is wounded it gives off certain signals. In response to those signals, A.T becomes activated and directs a series of events required for the transfer of T-dna from ti plasmid to the plant chromosome. The function of different vir gene includes a copy of T-dna followed by attachment of product to the copied T-dna strand, subsequently add proteins along with the length of T-dna, and possibly act as a protective mechanisms. These eventually open a channel...