Blotting and Hybridization Techniques-Applications
Applications of Blotting and Hybridization Techniques 1. Southern blotting technique is widely used to find specific nucleic acid sequence present in different plant species. 2. Northern blotting technique is widely used to find gene expression and regulation of specific genes. 3. By using blotting technique we can identify infectious agents present in the sample. 4. We can identify inherited disease. 5. It can be applied to mapping restriction sites in single copy gene. Disadvantages of Blotting and Hybridization Techniques 1. The process is a complex, cumbersome and time consuming one. 2. It requires electrophoretic separation. 3. Only one gene or RNA can be analysed at a time. 4. Gives information about presence of DNA, RNA or proteins but does not give information about regulation and gene interaction. Dot Blotting Techniques - The drawbacks of blotting techniques have lead to the developme...